Vacuum Pump

Vac Elut 12 and 20 Position Manifold Agilent Technologies

The Agilent 12- and 20-position extraction manifold features :

12- or 20-place positions for luer tip SPE cartridges

Simple tip design allows for easy use of stopcocks or disposable needle tips

Vacuum gauge with control and release valves for monitoring and adjustment of vacuum level

A choice of four racks to accommodate a variety of collection vessels

Three-point interlocking system to ensure proper orientation of racks, keying cartridge position to corresponding collection vessel

Chemically resistant, non-contaminating construction of glass, polyethylene, polypropylene, and Delrin components

Individually controlled, needle-tipped valves ( stopcocks ) permit flow control of each position

Identifying Parts

Before assembling the extraction manifold, ensure that you have these parts, pictured in Figure 1.

Glass chamber with exit valve installed

White Delrin cover* with polyethylene gasket, vacuum gauge, control and release valves installed

Disposable needle tips, long ( polypropylene )

Stopcock valves

Luer plugs, male

Ejector tool

Rack, 12- or 20- position, as specified

*The three posts on the underside of the cover serve as elevated feet so the cover can be set down without damaging the needle tips. They also maintain specific alignment of sample cartridge and collection vessel.

Using the Extraction Manifold

A typical solid phase extraction ( SPE ) procedure consists of the following steps :

1. Condition / equilibrate the SPE column bed.

2. Load the sample.

3. Wash off interferences.

4. Elute analyte ( s ) of interest.

The Agilent extraction manifold permits these steps to be carried out on up to twenty cartridges in parallel.

When sending all the eluents to waste, a rack is not necessary. This is usually the case for a typical SPE protocol when performing steps 1 through 3 above. In these cases waste is typically drawn into a liquid trap through the manifold’s exit valve located at the base of the glass chamber. When optimizing or troubleshooting an SPE protocol, the waste from these steps can be collected in tubes located in the racks for individual analys

Set up a vacuum system

1. Connect the outlet side of a liquid trap to a vacuum source using vacuum tubing.CAUTION : Make certain that the liquid trap is compatible with, and has sufficient capacity to collect all the waste that will be generated in your SPE procedure. If the trap capacity is too small, waste may contaminate and /or damage the vacuum pump.

2. Connect the manifold’s exit valve at the base of the glass chamber to the trap inlet using ⅜ in id vacuum tubing.

3. Put the cover top on the glass chamber. Do not install a rack at this time unless the needle tips and stopcocks required for your protocol are securely mounted in the top cover.

Installing needle tips and stopcock valves

Install a stopcock with the valve handle pointing outward or a disposable needle tip in each of the top cover ports as detailed in this procedure and as shown in Figure 1. This makes the valve accessible for operation. The

tips of the needles and stopcocks will extend into collection tubes mounted in a rack inside the manifold.

CAUTION : You must follow this procedure to obtain proper sealing of components mounted in the top cover. Failure to follow this procedure will cause joint leaks resulting in vacuum loss.

1. If installed, remove the rack from the manifold.

2. Place the top cover on the manifold and be sure it is properly seated on the edge of the glass chamber. CAUTION : Damage to the top cover or rack may result if the rack is left in the manifold and the three alignment posts are resting on the rack when pressure is applied to the top cover. If the rack must be left in the manifold, verify the top cover is resting on the glass chamber and not on the rack before proceeding.

3. Insert a needle tip into a hexagonal hole and lightly wiggle the needle while pushing it down inside the hexagonal hole. Using your thumb, push the needle as far as it can go. Repeat this step for the rest of the needle tips. Use this same method to install stopcocks.

4. Place an ejector handle horizontally on top of two needles and press down hard on it. Repeat this procedure on all of the needles. Also use this procedure to seal the stopcock joint to the top cover.

5. For needle tips that are not in use, install a luer plug by pushing it down in the needle tip’s inlet hole. The luer plug is used only with needle tips.

DO NOT INSTALL LUER PLUGS DIRECTLY ON THE COVER!

Removing items from the top cover

1. Remove all liquid from the columns.

2. To remove a luer plug or column, first twist it to break the friction joint. When the part turns freely, lift it out. CAUTION : Do not pull a plug or column with a tight connection straight upward. This force can cause the joint between the top cover and the needle tip or stopcock to loosen, resulting in a loss of vacuum.

3. To remove a needle tip and/or stopcock valve, pry it out carefully with the ejector tool. CAUTION : Push the ejector tool down slowly to prevent the launching of the needle tip.

Remove or install a rack of collection tubes

1. Close the exit valve or, alternatively, turn off the vacuum pump.

2. Break the vacuum in the manifold chamber by slightly offsetting the white ball from its seat ( see Figure 2 ).

3. Remove the cover when the vacuum gauge reads atmospheric pressure.

4. Install or remove a rack of tubes.

5. Replace the top cover. If a rack is installed, carefully align the three posts on the bottom of the cover with the corresponding notches on the top plate of the rack.

Manifold setup for protocol

1. Install the needle tips and stopcocks required by the protocol.

2. If required, remove or install a rack of tubes.

3. Put an extraction cartridge into each valve hub and seat it firmly.

4. Close all valves by turning the handles until each indicator shows that the valve is in the closed position.

5. Set up the vacuum system and turn on the vacuum pump. Adjust the check valve to set the desired vacuum level.

6. Follow the SPE protocol recommended for the type of column used and the application. Add fluid into the reservoirs before opening the stopcocks to start or resume flow.

7. Open each column’s stopcock valve to evacuate the liquid as required by the protocol.

Ordering Informationcription Part No.

Standard Vac Elut 20 Manifold

Vac Elut manifold with collection rack for 10 x 75 mm test tubes 12234105

Vac Elut manifold with collection rack for 13 x 75 mm test tubes 12234100

Vac Elut manifold with collection rack for 13 x 100 mm test tubes 12234101

Vac Elut manifold with collection rack for 16 x 75 mm test tubes 12234102

Vac Elut manifold with collection rack for 16 x 100 mm test tubes 12234103

Racks and replacement parts for Standard Vac Elut 20 Manifold

Standard glass basin 12234505

20-port collection rack for 13 x 75 mm test tubes 12234507

20-port collection rack for 13 x 100 mm test tubes 12234508

20-port collection rack for 16 x 75 mm test tubes 12234509

20-port collection rack for 16 x 100 mm test tubes 12234510

Replacement lid gasket 12234502

Vac Elut 20 lid cover 12234501

Tall Vac Elut 20 Manifold

Vac Elut 20 manifold with tall glass basin and collection rack for 16 x 150 mm test tubes, complete system 12234104

20-port Collection rack for 10 x 75 mm test tubes 12234517

Tall glass basin 12234512

Vac Elut 12 Manifold

12-port vacuum manifold processing station and collection rack for 16 x 100 mm test tubes 5982-9110

Racks and replacement parts for Vac Elut 12 Manifolds

Vac Elut 12 lid cover 5982-9111

Vac Elut 12 sealing gasket 5982-9112

Vac Elut 12 glass chamber 5982-9113

12-port collection rack for 13 x 75 mm test tubes 5982-9114

12-port collection rack for 13 x 100 mm test tubes 5982-9115

12-port collection rack for 16 x 75 mm test tubes 5982-9116

12-port collection rack for 16 x 100 mm test tubes 5982-9117

Common parts and disposables for all Manifolds

Manifold needle tip ejector tool 5982-9105

Polypropylene delivery needles 25/pk 12234511

Manifold SS needle with polypropylene coating 25/pk 12234042

Stainless steel needle tip 25/pk 12234038

Replacement exit valve for glass basin 12234506

Manifold exit valve replacement kit 12234005

Vacuum gauge assembly 12234504

Manifold ball ring/vacuum quick release 12234013

Manifold short valve stopcock 15/pk 12131005

Manifold long valve stopcock 20/pk 12234520

Manifold male luer plugs 25/pk 12234518